panel characteristics performance parameters tp53 mastr the tp53 mastr is a research panel for the identification of variants in the coding region of tp53 , a gene frequently mutated in a range of cancers. research application ? for detection of mutations (snvs and small indels) in tp53 in ffpe-derived dna ? for detection of mutations (snvs and small indels) in tp53 in blood-derived dna ? for detection of mutations (snvs and small indels) in tp53 in fft-derived dna gene full coding region of tp53 (excl. promoter 5 or 3 utr) genomic region analyzed 2.4 kb number of amplicons 17 amplicon length 176 - 260 bp number of plexes 2 design compatible with miseq, nextseq, miniseq and ion pgm systems number of samples/run @ 5 % vafsample: illumina miseq v2: 211* illumina miseq v3: 388* tp53 * only 192 mid combinations available. due to the low complexity of the library, it is advised to combine with other assays on a sequencing run ** number of samples per run for illumina & iontorrent ngs systems can be calculated via the sequencing calculator. uniformity of amplifcation ( 0.2 x mean coverage) 99 % amplicons on high-molecular weight dna 96 % amplicons detected on dna from ffpe tissue on target read count >93 % for ffpe-derived dna low dna amount 4 ng per plex reaction lowest observed mutation frequency 3 % 17
? agilent technologies, inc. 2017 printed in belgium, december 31, 2017 5991-8391enn pr7000-1416 workflow order information cat. no. product name samples mr-0160.024 tp53 mastr 24 mid (molecular identifiers) kits are necessary to complete the workflow. for research use only. not for use in diagnostic procedures. plan run multiplex pcr quality control universal pcr quality control equimolar pooling mixing plexes of single sample puri? cation multiplex pcr dilution universal pcr mixing clean up pooling sequencing data analysis mastr work? ow ngs multiplicom n.v. [ a ] galile?laan 18, b-2845 niel, belgium [ w ] www.agilent.com [ e ] mtp.customerservice@agilent.com [ t ] +32 3 289 14 00 [ f ] +32 3 400 23 16
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